Purification and Characterization of a Prolyl Aminopeptidase from Debaryomyces hansenii
نویسندگان
چکیده
منابع مشابه
Purification and characterization of a prolyl aminopeptidase from Debaryomyces hansenii.
A prolyl aminopeptidase (PAP) (EC 3.4.11.5) was isolated from the cell extract of Debaryomyces hansenii CECT12487. The enzyme was purified by selective fractionation with protamine and ammonium sulfate, followed by two chromatography steps, which included gel filtration and anion-exchange chromatography. The PAP was purified 248-fold, with a recovery yield of 1.4%. The enzyme was active in a br...
متن کاملLipids of Debaryomyces Hansenii.
Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Edward M. Devine, Jr. Lipids of Debaryomyces hansenii. J. Bacteriol. 89:1488-1493. 1965.-The separation of neutral lipids and phospholipids from the lipid extract of Debaryomyces hansenii NRRL Y-1448 was accomplished by using a single column packed with silicic acid. The neutral lipids comprised 67%, and the phospholipids 33%, of the...
متن کاملPurification and Partial Characterization of a Prolyl-Dipeptidyl Aminopeptidase from Lactobacillus helveticus CNRZ 32.
X-prolyl-dipeptidyl aminopeptidase, which hydrolyzed Gly-Pro-p-nitroanilide (relative activity [RA] = 100%) and Arg-Pro-p-nitroanilide (RA, 130%), was purified to homogeneity from the cell extract of Lactobacillus helveticus CNRZ 32. The enzyme also hydrolyzed Ala-Pro-Gly (RA, 11%) and Ala-Ala-p-nitroanilide (RA, 2%) but was not active on Ala-Leu-Ala, dipeptides, and endopeptidase and carboxype...
متن کاملPurification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa).
We have found a novel prolyl aminopeptidase in Grifola frondosa. The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylm...
متن کاملCharacterization of New Polyol/H+ Symporters in Debaryomyces hansenii
Debaryomyces hansenii is a halotolerant yeast that produces and assimilates a wide variety of polyols. In this work we evaluate polyol transport in D. hansenii CBS 767, detecting the occurrence of polyol/H(+) (and sugar/H(+)) symporter activity, through the transient extracellular alkalinization of unbuffered starved cell suspensions. From the D. hansenii genome database, we selected nine ORFs ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.1.227-232.2003